Biosafety Manual

Directory of Service and Emergency Providers 3
A. Introduction 8
Definition of Biohazardous Materials 8
Purpose 8
Responsibilities 9
Iowa State University (ISU) 9
Environmental Health and Safety (EH&S) 9
Supervisors 9
Personnel 9
Institutional Biosafety Committee (IBC) 9
C. Medical Survelliance 13
Workplace Exposure Assessment 13
Exposure Monitoring 13
Vaccinations and Testing 14
Exposure to Biohazardous Materials 14
Work-Related Injuries, Illnesses and Exposures 15
Reporting 15
Student Accidents and Injuries 15
D. Biosafety Practices and Procedures 17
Work Practices (First Line Of Defense) 17
Laboratory Biosafety Level Criteria 17
Biosafety Level 1 (BSL–1) Minimum Criteria 19
Biosafety Level 2 (BSL–2) Minimum Criteria 20
Biosafety Level 3 (BSL–3) Minimum Criteria 21
Biosafety Level 4 (BSL–4) 22
Laboratory Decommissioning 23
Training and Education 23
Signs and Labeling 23
Security 24
Personal Protective Equipment 24
Laboratory Practice and Technique 26
Pipetting 27
Centrifugation 28
Using Needles, Syringes and Other Sharps 29
Blending, Grinding, Sonicating, Lyophilizing, and Freezing 29
Open Flames 30
Flow Cytometry 30
Evaluating Laboratory Safety 31
Animal Handling 31
Animals on Campus 31
Animals in the Field 32
Arthropod Research 33
Arthropod Containment Levels 33
Cell and Tissue Culture 35
Safety Equipment (Primary Containment) 36
Biosafety Cabinets (BSC) 37
What is a Biosafety Cabinet? 37
When Must I Use a BSC? 37
Open Flames in a BSC 37
Decontamination and Ultraviolet Lights in a BSC 37
Annual Certification Testing 38
Moving or Repairs 38
Purchasing and Installing a New BSC 38
Facility Design (Secondary Containment) 39
E. Disposal and Disinfection of Biohazardous Materials 41
University Policies 41
Supplies 41
What If I Do Not Have Waste Handling Facilities? 41
Autoclaves 41
Elements Required for Effective Autoclave Use 41
Autoclave Safety 43
Pressure Vessel Monitoring 43
Chemical Disinfectants 44
Choosing a Chemical Disinfectant 44
Types of Chemical Disinfectants 45
Procedures For Inactivation and Safe Containment of Toxins 48
Prion Inactivation and Biocontainment Procedures 48
USDA Recommendations for Inactivation of Prions Affecting Livestock 48
BMBL Recommendations for Inactivation of Prions Affecting Humans 48
Precautions in Using NaOH or Sodium Hypochlorite Solutions in Autoclaves 49
Biocontainment and Working Procedures 49
F. Biohazard Spill Clean-up 50
Biohazard Spill Kit 50
Biohazard Spill Response Procedure 51
G. Transporting and Shipping Biohazardous Materials 53
On-Campus Transport of Biohazardous Materials 53
Off-Campus Transport of Biohazardous Materials by Commercial Carriers 53
Permit Requirements 54
Animals, Plants, Introduction of Genetically Modified Organisms 54
Human Pathogens or Biological Toxins 54
CDC Importation Permits for Etiologic Agents 54
Select Agents and Toxins (SAT) 55
Packaging and Paperwork Requirements 56
Off-Campus Transport of Biohazardous Materials by Non-Commercial Routes 57
H. Biological Material Inventory and Biohazardous Materials Security 59
Bioinventory 59
Biosecurity 59
Non-discrimination Statement 60
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