Biosafety Manual

A. INTRODUCTION 7
Definition of Biohazardous Materials 7
Purpose 7
Responsibilities 8
Exposure to Biohazardous Materials 13
Vaccinations and Testing 13
Biosafety Cabinets 35
Safety Equipment (Primary Containment) 35
Arthropod Research 32
Animal Handling 30
Evaluating Laboratory Safety 30
Personal Protective Equipment 23
Signs and Labeling 22
Training and Education 22
Laboratory Decommissioning 22
Biosafety Level 4 (BSL-4) 21
Biosafety Level 3 (BSL-3) Minimum Criteria 20
Biosafety Level 2 (BSL-2) Minimum Criteria 19
Biosafety Level 1 (BSL-1) Minimum Criteria 18
Laboratory Biosafety Level Criteria 16
Work Practices (First Line Of Defense) 16
Laboratory Practice and Technique 25
Prion Inactivation and Biocontainment Procedures 47
Procedures For Inactivation and Safety Containment of Toxins 47
Chemical Disinfectants 43
Autoclaves 40
What If I Do Not Have Waste Handling Facilities? 40
Supplies 40
University Policies 40
F. BIOHAZARD SPILL CLEAN-UP 48
Biohazard Spill Kit 49
Biohazard Spill Clean-Up Protocol 50
Off-Campus Transport of Biohazardous Materials by Commercial Carriers 52
On-Campus Transport of Biohazardous Materials 52
Bioinventory 58
Directory of Service and Emergency Providers 3
A. Introduction 7
Definition of Biohazardous Materials 7
Purpose 7
Responsibilities 8
Iowa State University (ISU) 8
Environmental Health and Safety (EH&S) 8
Supervisors 8
Personnel 8
Institutional Biosafety Committee (IBC) 8
C. Medical Survelliance 12
Workplace Exposure Assessment 12
Exposure Monitoring 12
Vaccinations and Testing 13
Exposure to Biohazardous Materials 13
Work-Related Injuries, Illnesses and Exposures 14
Reporting 14
D. Biosafety Practices and Procedures 16
Work Practices (First Line Of Defense) 16
Laboratory Biosafety Level Criteria 16
Biosafety Level 1 (BSL–1) Minimum Criteria 18
Biosafety Level 2 (BSL–2) Minimum Criteria 19
Biosafety Level 3 (BSL–3) Minimum Criteria 20
Biosafety Level 4 (BSL–4) 21
Laboratory Decommissioning 22
Training and Education 22
Signs and Labeling 22
Security 23
Personal Protective Equipment 23
Laboratory Practice and Technique 25
Pipetting 26
Centrifugation 27
Using Needles, Syringes and Other Sharps 27
Blending, Grinding, Sonicating, Lyophilizing, and Freezing 28
Open Flames 29
Flow Cytometry 29
Evaluating Laboratory Safety 30
Animal Handling 30
Animals on Campus 30
Animals in the Field 31
Arthropod Research 32
Arthropod Containment Levels 32
Cell and Tissue Culture 34
Safety Equipment (Primary Containment) 35
Biosafety Cabinets (BSC) 36
What is a Biosafety Cabinet? 36
When Must I Use a BSC? 36
Open Flames in a BSC 36
Decontamination and Ultraviolet Lights in a BSC 36
Annual Certification Testing 37
Moving or Repairs 37
Purchasing and Installing a New BSC 37
Facility Design (Secondary Containment) 38
E. Disposal and Disinfection of Biohazardous Materials 40
University Policies 40
Supplies 40
What If I Do Not Have Waste Handling Facilities? 40
Autoclaves 40
Elements Required for Effective Autoclave Use 40
Autoclave Safety 42
Pressure Vessel Monitoring 42
Chemical Disinfectants 43
Choosing a Chemical Disinfectant 43
Types of Chemical Disinfectants 44
Procedures For Inactivation and Safe Containment of Toxins 47
Prion Inactivation and Biocontainment Procedures 47
USDA Recommendations for Inactivation of Prions Affecting Livestock 47
BMBL Recommendations for Inactivation of Prions Affecting Humans 47
Precautions in Using NaOH or Sodium Hypochlorite Solutions in Autoclaves 48
Biocontainment and Working Procedures 48
F. Biohazard Spill Clean-up 49
Biohazard Spill Kit 49
Biohazard Spill Response Procedure 50
G. Transporting and Shipping Biohazardous Materials 52
On-Campus Transport of Biohazardous Materials 52
Off-Campus Transport of Biohazardous Materials by Commercial Carriers 52
Permit Requirements 53
Animals, Plants, Introduction of Genetically Modified Organisms 53
Human Pathogens or Biological Toxins 53
CDC Importation Permits for Etiologic Agents 53
Select Agents and Toxins (SAT) 54
Packaging and Paperwork Requirements 55
Off-Campus Transport of Biohazardous Materials by Non-Commercial Routes 56
H. Biological Material Inventory and Biohazardous Materials Security 58
Bioinventory 58
Biosecurity 58
Non-discrimination Statement 59
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