Biosafety Manual 17 BIOSAFETY LEVELS - THE SHORT VERSION (adapted from BMBL with Iowa State University policies incorporated) BSL Agents Practices Safety Equipment (Primary Barriers) Facilities (Secondary Barriers) 1 Well-characterized agents not known to consistently cause disease in immunocompetent adult humans and present minimal potential hazard to laboratory personnel and the environment. Standard microbiological practices Refer to Iowa State University policy on minimum personal protective equipment (PPE) for labs: lab coats, gloves, eye and/or face protection Open bench top Hand-washing sink required 2 Associated with human disease Hazard: percutaneous injury, ingestion, mucous membrane exposure BSL-1 practice plus: Biosafety Cabinets (BSCs) Leak-proof container for sample transport Primary barriers: Class I or II Biosafety Cabinets or other physical containment devices used for all manipulations of agents that may cause splashes or aerosols of infectious materials PPE: lab coat, gloves, eye and/or face protection BSL-1 plus: Autoclave available 3 Indigenous or exotic agents with potential for aerosol transmission; disease may have serious or lethal consequences BSL-2 practice plus: Controlled access Decontamination of all wastes Decontamination of lab clothing before laundering Baseline serum Primary barriers: Class I or II BSCs or other physical containment devices used for all open manipulations of agents PPE: protective lab clothing, gloves, respiratory, eye and/ or face protection BSL-2 plus: Physical separation from access corridors Self-closing, double door access Exhaust air not recirculated Directional airflow into laboratory 4 Materials requiring BSL-4 facilities and practices are not used at Iowa State University. Materials requiring BSL-4 facilities and practices are not used at Iowa State University. Materials requiring BSL4 facilities and practices are not used at Iowa State University. Materials requiring BSL4 facilities and practices are not used at Iowa State University. Reference: BMBL, current edition and NIH Guidelines Animal biosafety levels describe similar levels for containment facilities and practices necessary when vertebrate animals are infected with human pathogens (ABSL-2, ABSL-3, ABSL-4). Plant biosafety levels describe similar levels for greenhouse containment facilities and practices necessary for recombinant plants and plants infected with plant pathogens or plant pests (BL1-P, BL2-P, BL3-P, BL4-P). Most laboratories on campus qualify as BSL-1 or BSL-2. Four ABSL-3/BSL-3 facilities serve the ISU research community.