Biosafety Manual

18 Biosafety Manual The following lists summarize the minimum criteria for laboratories operating at Biosafety Levels 1-3. These criteria are detailed in the current edition of the Biosafety in Microbiological and Biomedical Laboratories (BMBL), a joint publication of the CDC and NIH. Biosafety Level 1 (BSL–1) Minimum Criteria • Personnel are trained in analytical methods, standard operating procedures (SOPs), spill response, potential hazards, and applicable safety training. • Access to the laboratory is limited or restricted at the discretion of the laboratory director when work with cultures or specimens is in progress. • Laboratory walls, floors, and ceilings are designed to be easily cleaned. • Bench tops are impervious to water and resistant to heat, acids, bases, and solvents. • Laboratory furniture is appropriate for use and easily accessible for cleaning. • Laboratory windows are fitted with insect screens. • Laboratory lighting must be adequate for all activities. • Laboratory is outfitted with a hand-washing sink with soap and towels. • Appropriate PPE is used in the laboratory (for example lab coats, safety glasses/goggles, closed-toe shoes, and gloves). • Where research animals are present in the laboratory the risk assessment considers appropriate eye, face, and respiratory protection, as well as potential animal allergens. • Laboratory personnel are enrolled in the respiratory protection program when warranted by risk assessment. • Only animals or plants associated with current studies are present in the laboratory. • Personnel wash their hands after handling biohazardous agents or animals, after removing gloves, or before leaving the room. • Eating, drinking, smoking, applying cosmetics, handling contact lenses, and storing food for human consumption are prohibited in the laboratory. • Mouth pipetting is prohibited. Mechanical devices are used for pipetting. • Policy is in place for safe handling of sharps.

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