Biosafety Manual

19 Biosafety Manual • First aid kit is accessible and suitably stocked. • Procedures are in place to minimize splashes and the creation of aerosols. • Work surfaces are decontaminated with an appropriate disinfectant at least once a day and after a spill of viable material. • Laboratory equipment is decontaminated routinely and after contamination; and before repair, maintenance, or removal from the laboratory. • Cultures, stocks, and regulated waste are decontaminated by an effective method before disposal. • Proper biological waste labeling is in place for off-site decontamination. • Appropriate biohazard containers are used for containment of biohazardous waste. • Laboratory has a rodent and pest control program in place. • A biohazard sign indicating the required biosafety level, required PPE, exit procedures, required immunizations, and the PI’s name and phone number is posted at the laboratory entrance during work with human pathogens. Biosafety Level 2 (BSL–2) Minimum Criteria • BSL-1 minimum criteria are followed. • An autoclave is available for decontaminating laboratory wastes. • Personnel have been assessed to determine if experimental work poses any special risks to the individual. • Personnel have been trained in the specific hazards associated with pathogenic agents and Recombinant or Synthetic Nucleic Acid Molecules in accordance with SOPs and protocols. Training will be documented and available for inspection. • At risk laboratory personnel have received appropriate immunizations if available. • Laboratory personnel have submitted a baseline serum sample when appropriate. • A biosafety manual and SOPs are written to incorporate specific biosafety precautions appropriate to the laboratory. • Procedures for handling a spill or accident, including required follow-up and documentation, are in place and available to lab personnel. • Exhaust HEPA filters or equipment containing HEPA filters, including biosafety cabinets and cage racks, must be certified

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