21 Biosafety Manual required for air exiting the room to the outdoors. • Directional airflow, flowing from clean areas to contaminated areas, is provided. • All experimental manipulations are done inside a certified biosafety cabinet (primary containment). • Every consideration is given to alternative forms of needles or glassware to prevent sharps injuries. • Equipment exposed to BSL-3 agents is decontaminated before any repair, service or disposal. • Protective clothing consists of solid-front gowns, scrub suits, or coveralls. • Respiratory and face protection is used when in rooms containing infected animals. • A hands-free sink with soap and paper towels is available for use near the exit door. • If present, all windows are closed and sealed. • All cultures, stocks, biological waste, gloves, gowns, and other contaminated articles are decontaminated in the laboratory. • Vacuum lines are protected with liquid disinfectant traps and HEPA filters. • All procedures for the facility designandoperationaredocumented. • The BSL-3 facility design, operational parameters, and procedures are verified and documented prior to operation. • The BSL-3 facility is tested annually or after significant modification to ensure operational parameters are met. Verification criteria are modified as necessary by operational experience. • Exhaust HEPA filters and equipment containing HEPA filters must be certified annually. Biosafety Level 4 (BSL–4) Use of materials requiring BSL-4 facilities and practices must be conducted within a certified BSL4 facility. Additional Resources U.S. Department of Agriculture (USDA) Regulations for Animals and Animal Products (9 CFR 001-199) • Import/transport permits are issued by theAnimal and Plant Health Inspection Service (APHIS) Veterinary Services (VS) branch • Quick reference and applications for import and interstate
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