Biosafety Manual

47 Biosafety Manual final concentration, then rinsed with water and autoclaved at 121C (gravity displacement) or 134C (porous load) for 1 hour. • Carefully package contaminated materials and incinerate at >1,000°C. • Non-disposable instruments with gross contamination removed should be soaked in 2N sodium hydroxide or sodium hypochlorite (20,000 ppm) for 1 hour. Precautions in Using NaOH or Sodium Hypochlorite Solutions in Autoclaves Sodium hydroxide spills or gas may damage the autoclave if proper containers are not used. The use of containers with a rim and lid designed for condensation to collect and drip back into the pan is recommended. Persons who use this procedure should be cautious in handling hot sodium hydroxide solution (post-autoclave) and in avoiding potential exposure to gaseous sodium hydroxide, exercise caution during all sterilization steps, and allow the autoclave, instruments, and solutions to cool down before removal. Biocontainment and Working Procedures • Utilize a Class II biosafety cabinet for all manipulations of samples. • Utilize personal protective equipment, including nitrile gloves, lab coat, and eye protection. • Use disposable instruments (scalpels, pipettes, etc.) when possible. Refer to the current edition of BMBL for additional information and recommendations regarding work with and disposal of prions. Additional Resources U.S. Environmental Protection Agency (EPA) Hospital/Medical/ Infectious Waste Incinerators Regulations (40 CFR 62) • Emissions requirements for hospital, medical, and infectious waste incinerators • Iowa State University Sharps and Biohazardous Waste Policy