Biosafety Manual

48 Biosafety Manual F. Biohazard Spill Clean-up The following protocol is generic and is intended for use with microorganisms worked with at BSL-2 or lower. The correct protocol for any situation depends on the specific biohazardous material used, quantity of material spilled, and location of the spill. All spills in the laboratory should be reported immediately to the supervisor in charge of the lab. Questions about spill clean-up or the use of organisms worked with at BSL-3 should be directed to Environmental Health and Safety (EH&S) biosafety staff; call (515) 294-5359. If a biohazardous spill also includes radioactive material, the clean- up protocol may need to be modified. For these situations, contact the Radiation Safety Officer at (515) 294-5359 during the regular workday. The Department of Public Safety should be contacted at (515) 294-4428 for spill clean-up questions after hours. Biohazard Spill Kit Each laboratory using biohazardous materials (recombinant or synthetic nucleic acid molecules, synthetic molecules, animal pathogens, human pathogens, and plant pathogens) must have appropriate equipment and supplies on hand for managing spills and accidents involving biohazardous materials. Permanent equipment should include a safety shower, eyewash, and a hand-washing sink. A Biohazard Spill Kit should be available in the areas where work is being conducted with biohazardous materials. The supplies available in a Biohazard Spill Kit should include, but are not limited to: • A copy of the following Biological Spill Cleanup Procedures • Nitrile disposable gloves (8 mil)(check for holes or deterioration; replace box of nitrile gloves every two years) • Lab coat(s) or gowns • Goggles or safety glasses with side shields • Face masks • Disposable shoe covers (booties) • Absorbent material, such as absorbent paper towels, granular absorbent material, etc. (a disposable or cleanable scoop will be needed for granular absorbent) • All-purpose disinfectant, such as normal household bleach (freshly diluted 1:10), an iodophor (such as Wescodyne), or a quaternary ammonia preparation (such as EndBac II) • Autoclavable bucket for diluting disinfectant (this can be used to store the kit contents when not in use)

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