Biosafety Manual 5 Security 23 Personal Protective Equipment (PPE) 24 Laboratory Practice and Technique 26 Pipetting 27 Centrifugation 28 Using Needles, Syringes, and Other Sharps 28 Blending, Grinding, Sonicating, Lyophilizing, and Freezing 29 Open Flames 29 Flow Cytometry 30 Evaluating Laboratory Safety 30 Animal Handling 31 Animals on Campus 31 Animals in the Field 32 Arthropod Research 32 Arthropod Containment Levels 33 Cell and Tissue Culture 34 Safety Equipment (Primary Containment) 35 Biosafety Cabinets (BSC) 35 What is a Biosafety Cabinet? 35 When Must I Use a BSC? 36 Open Flames in a BSC 36 Decontamination and Ultraviolet Lights in a BSC 36 Annual Certification Testing 37 Moving or Repairs 37 Purchasing and Installing a New BSC 37 Facility Design (Secondary Containment) 38 E. Disposal and Disinfection of Biohazardous Materials 39 University Policies 39 Supplies 39 What If I Do Not Have Waste Handling Facilities? 39
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