Biosafety Manual 6 Autoclaves 39 Elements Required for Effective Autoclave Use 39 Autoclave Safety 41 Pressure Vessel Monitoring 41 Chemical Disinfectants 42 Choosing a Chemical Disinfectant 42 Types of Chemical Disinfectants 43 Inactivation, Verification, and Biocontainment of Infectious Agents and Toxins 46 Prion Inactivation and Biocontainment Procedures 46 USDA Recommendations for Inactivation of Prions Affecting Livestock 46 BMBL Recommendations for Inactivation of Prions Affecting Humans 46 Precautions in Using NaOH or Sodium Hypochlorite Solutions in Autoclaves 47 Biocontainment and Working Procedures 47 F. Biohazard Spill Clean-up 48 Biohazard Spill Kit 48 Biohazard Spill Response Procedure 49 G. Transporting and Shipping Biohazardous Materials 51 On-Campus Transport of Biohazardous Materials 51 Off-Campus Transport of Biohazardous Materials by Commercial Carriers 51 Permit Requirements 52 Animals, Plants, Introduction of Genetically Modified Organisms 52 Human Pathogens or Biological Toxins 52 CDC Importation Permits for Etiologic Agents 52 Select Agents and Toxins 53 Packaging and Paperwork Requirements 54 Off-Campus Transport of Biohazardous Materials by Non-Commercial Routes 55 H. Biological Material Inventory and Biohazardous Materials Security 57 Bioinventory 57 Biosecurity 57 Non-discrimination Statement 58
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