| Directory of Service and Emergency Providers |
3 |
| A. Introduction |
7 |
| Definition of Biohazardous Materials |
7 |
| Purpose |
7 |
| Responsibilities |
8 |
| Iowa State University (ISU) |
8 |
| Environmental Health and Safety (EH&S) |
8 |
| Supervisors |
8 |
| Personnel |
8 |
| Institutional Biosafety Committee (IBC) |
8 |
| B. Recombinant or Synthetic Nucleic Acid Molecules, Human, Animal, and Plant Pathogens, Biological Toxins: Institutional Biosafety Committee (IBC) |
10 |
| C. Medical Survelliance |
12 |
| Workplace Exposure Assessment |
12 |
| Exposure Monitoring |
12 |
| Vaccinations and Testing |
13 |
| Exposure to Biohazardous Materials |
13 |
| Work-Related Injuries, Illnesses and Exposures |
14 |
| Reporting |
14 |
| D. Biosafety Practices and Procedures |
16 |
| Work Practices (First Line Of Defense) |
16 |
| Laboratory Biosafety Level Criteria |
16 |
| Biosafety Level 1 (BSL–1) Minimum Criteria |
18 |
| Biosafety Level 2 (BSL–2) Minimum Criteria |
19 |
| Biosafety Level 3 (BSL–3) Minimum Criteria |
20 |
| Biosafety Level 4 (BSL–4) |
21 |
| Laboratory Decommissioning |
22 |
| Training and Education |
22 |
| Signs and Labeling |
23 |
| Security |
23 |
| Personal Protective Equipment (PPE) |
24 |
| Laboratory Practice and Technique |
26 |
| Pipetting |
27 |
| Centrifugation |
28 |
| Using Needles, Syringes, and Other Sharps |
28 |
| Blending, Grinding, Sonicating, Lyophilizing, and Freezing |
29 |
| Open Flames |
29 |
| Flow Cytometry |
30 |
| Evaluating Laboratory Safety |
30 |
| Animal Handling |
31 |
| Animals on Campus |
31 |
| Animals in the Field |
32 |
| Arthropod Research |
32 |
| Arthropod Containment Levels |
33 |
| Cell and Tissue Culture |
34 |
| Safety Equipment (Primary Containment) |
35 |
| Biosafety Cabinets (BSC) |
35 |
| What is a Biosafety Cabinet? |
35 |
| When Must I Use a BSC? |
36 |
| Open Flames in a BSC |
36 |
| Decontamination and Ultraviolet Lights in a BSC |
36 |
| Annual Certification Testing |
37 |
| Moving or Repairs |
37 |
| Purchasing and Installing a New BSC |
37 |
| Facility Design (Secondary Containment) |
38 |
| E. Disposal and Disinfection of Biohazardous Materials |
39 |
| University Policies |
39 |
| Supplies |
39 |
| What If I Do Not Have Waste Handling Facilities? |
39 |
| Autoclaves |
39 |
| Elements Required for Effective Autoclave Use |
39 |
| Autoclave Safety |
41 |
| Pressure Vessel Monitoring |
41 |
| Chemical Disinfectants |
42 |
| Choosing a Chemical Disinfectant |
42 |
| Types of Chemical Disinfectants |
43 |
| Inactivation, Verification, and Biocontainment of Infectious Agents and Toxins |
46 |
| Prion Inactivation and Biocontainment Procedures |
46 |
| USDA Recommendations for Inactivation of Prions Affecting Livestock |
46 |
| BMBL Recommendations for Inactivation of Prions Affecting Humans |
46 |
| Precautions in Using NaOH or Sodium Hypochlorite Solutions in Autoclaves |
47 |
| Biocontainment and Working Procedures |
47 |
| F. Biohazard Spill Clean-up |
48 |
| Biohazard Spill Kit |
48 |
| Biohazard Spill Response Procedure |
49 |
| G. Transporting and Shipping Biohazardous Materials |
51 |
| On-Campus Transport of Biohazardous Materials |
51 |
| Off-Campus Transport of Biohazardous Materials by Commercial Carriers |
51 |
| Permit Requirements |
52 |
| Animals, Plants, Introduction of Genetically Modified Organisms |
52 |
| Human Pathogens or Biological Toxins |
52 |
| CDC Importation Permits for Etiologic Agents |
52 |
| Select Agents and Toxins |
53 |
| Packaging and Paperwork Requirements |
54 |
| Off-Campus Transport of Biohazardous Materials by Non-Commercial Routes |
55 |
| H. Biological Material Inventory and Biohazardous Materials Security |
57 |
| Bioinventory |
57 |
| Biosecurity |
57 |
| Non-discrimination Statement |
58 |