25 Biosafety Manual When working with biohazardous materials inside a biosafety cabinet, elastic cuffs or double gloving (second pair over cuff) prevent contaminated air from being blown up the lab coat sleeve onto clothing. Gloves Gloves prevent exposure of the skin and any cuts, dermatitis, etc. that may be present, to biohazardous materials. • Both latex and nitrile disposable gloves will prevent exposure tomicroorganisms. However, nitrile glovesmust bewornwhen handling chemicals, since latex provides little to no protection from chemical exposure. EH&S laboratory personnel, can provide assistance with choosing appropriate gloves. • For best protection, the cuffs of the gloves should overlap the lower sleeves of the lab coat. • Consider the need for bite and/or scratch resistant gloves. • Disposable gloves must not be reused. They are designed for disposal after one use or if exposed to a chemical (they offer limited chemical protection). Dispose of used gloved with other contaminated laboratory waste. Utility gloves, such as rubber dish washing gloves, may be disinfected for re-use if they do not show signs of wear or degradation. • Change gloves when contaminated, when glove integrity is compromised, or when otherwise necessary. • For information concerning the chemical resistance of the different types of gloves, access the Ansell Chemical Resistance Guide. • EH&S can provide assistance with finding an alternative for personnel with allergic reactions to gloves (most common with latex) and/or the powder they contain. Eye and Face Protection Eye and face protection prevent splashes into the eyes, nose and mouth (mucous membrane exposure), and onto the skin. • Goggles or safety glasses must be worn when working with laboratory hazards. • Prescription safety eyewear is available through approved providers. • Face shields should be used for full face protection. • N-95 masks provide splash protection for the mouth and nose.