Directory of Service and Emergency Providers
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3 |
A. Introduction
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7 |
Definition of Biohazardous Materials
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7 |
Purpose
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7 |
Responsibilities
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8 |
Iowa State University (ISU)
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8 |
Environmental Health and Safety (EH&S)
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8 |
Supervisors
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8 |
Personnel
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8 |
Institutional Biosafety Committee (IBC)
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8 |
B. Recombinant or Synthetic Nucleic Acid Molecules, Human, Animal, and Plant Pathogens, Biological Toxins: Institutional Biosafety Committee (IBC)
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10 |
C. Medical Survelliance
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12 |
Workplace Exposure Assessment
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12 |
Exposure Monitoring
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12 |
Vaccinations and Testing
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13 |
Exposure to Biohazardous Materials
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13 |
Work-Related Injuries, Illnesses and Exposures
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14 |
Reporting
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14 |
D. Biosafety Practices and Procedures
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16 |
Work Practices (First Line Of Defense)
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16 |
Laboratory Biosafety Level Criteria
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16 |
Biosafety Level 1 (BSL–1) Minimum Criteria
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18 |
Biosafety Level 2 (BSL–2) Minimum Criteria
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19 |
Biosafety Level 3 (BSL–3) Minimum Criteria
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20 |
Biosafety Level 4 (BSL–4)
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21 |
Laboratory Decommissioning
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22 |
Training and Education
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22 |
Signs and Labeling
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23 |
Security
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23 |
Personal Protective Equipment (PPE)
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24 |
Laboratory Practice and Technique
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26 |
Pipetting
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27 |
Centrifugation
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28 |
Using Needles, Syringes, and Other Sharps
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28 |
Blending, Grinding, Sonicating, Lyophilizing, and Freezing
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29 |
Open Flames
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29 |
Flow Cytometry
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30 |
Evaluating Laboratory Safety
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30 |
Animal Handling
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31 |
Animals on Campus
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31 |
Animals in the Field
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32 |
Arthropod Research
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32 |
Arthropod Containment Levels
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33 |
Cell and Tissue Culture
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34 |
Safety Equipment (Primary Containment)
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35 |
Biosafety Cabinets (BSC)
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35 |
What is a Biosafety Cabinet?
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35 |
When Must I Use a BSC?
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36 |
Open Flames in a BSC
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36 |
Decontamination and Ultraviolet Lights in a BSC
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36 |
Annual Certification Testing
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37 |
Moving or Repairs
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37 |
Purchasing and Installing a New BSC
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37 |
Facility Design (Secondary Containment)
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38 |
E. Disposal and Disinfection of Biohazardous Materials
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39 |
University Policies
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39 |
Supplies
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39 |
What If I Do Not Have Waste Handling Facilities?
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39 |
Autoclaves
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39 |
Elements Required for Effective Autoclave Use
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39 |
Autoclave Safety
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41 |
Pressure Vessel Monitoring
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41 |
Chemical Disinfectants
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42 |
Choosing a Chemical Disinfectant
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42 |
Types of Chemical Disinfectants
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43 |
Inactivation, Verification, and Biocontainment of Infectious Agents and Toxins
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46 |
Prion Inactivation and Biocontainment Procedures
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46 |
USDA Recommendations for Inactivation of Prions Affecting Livestock
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46 |
BMBL Recommendations for Inactivation of Prions Affecting Humans
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46 |
Precautions in Using NaOH or Sodium Hypochlorite Solutions in Autoclaves
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47 |
Biocontainment and Working Procedures
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47 |
F. Biohazard Spill Clean-up
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48 |
Biohazard Spill Kit
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48 |
Biohazard Spill Response Procedure
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49 |
G. Transporting and Shipping Biohazardous Materials
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51 |
On-Campus Transport of Biohazardous Materials
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51 |
Off-Campus Transport of Biohazardous Materials by Commercial Carriers
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51 |
Permit Requirements
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52 |
Animals, Plants, Introduction of Genetically Modified Organisms
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52 |
Human Pathogens or Biological Toxins
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52 |
CDC Importation Permits for Etiologic Agents
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52 |
Select Agents and Toxins
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53 |
Packaging and Paperwork Requirements
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54 |
Off-Campus Transport of Biohazardous Materials by Non-Commercial Routes
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55 |
H. Biological Material Inventory and Biohazardous Materials Security
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57 |
Bioinventory
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57 |
Biosecurity
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57 |
Non-discrimination Statement
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58 |