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Directory of Service and Emergency Providers
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3 |
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A. Introduction
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7 |
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Definition of Biohazardous Materials
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7 |
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Purpose
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7 |
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Responsibilities
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8 |
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Iowa State University (ISU)
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8 |
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Environmental Health and Safety (EH&S)
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8 |
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Supervisors
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8 |
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Personnel
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8 |
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Institutional Biosafety Committee (IBC)
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8 |
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B. Recombinant or Synthetic Nucleic Acid Molecules, Human, Animal, and Plant Pathogens, Biological Toxins: Institutional Biosafety Committee (IBC)
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10 |
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C. Medical Survelliance
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12 |
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Workplace Exposure Assessment
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12 |
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Exposure Monitoring
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12 |
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Vaccinations and Testing
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13 |
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Exposure to Biohazardous Materials
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13 |
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Work-Related Injuries, Illnesses and Exposures
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14 |
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Reporting
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14 |
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D. Biosafety Practices and Procedures
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16 |
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Work Practices (First Line Of Defense)
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16 |
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Laboratory Biosafety Level Criteria
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16 |
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Biosafety Level 1 (BSL–1) Minimum Criteria
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18 |
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Biosafety Level 2 (BSL–2) Minimum Criteria
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19 |
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Biosafety Level 3 (BSL–3) Minimum Criteria
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20 |
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Biosafety Level 4 (BSL–4)
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21 |
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Laboratory Decommissioning
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22 |
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Training and Education
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22 |
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Signs and Labeling
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23 |
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Security
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23 |
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Personal Protective Equipment (PPE)
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24 |
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Laboratory Practice and Technique
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26 |
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Pipetting
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27 |
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Centrifugation
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28 |
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Using Needles, Syringes, and Other Sharps
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28 |
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Blending, Grinding, Sonicating, Lyophilizing, and Freezing
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29 |
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Open Flames
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29 |
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Flow Cytometry
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30 |
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Evaluating Laboratory Safety
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30 |
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Animal Handling
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31 |
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Animals on Campus
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31 |
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Animals in the Field
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32 |
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Arthropod Research
|
32 |
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Arthropod Containment Levels
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33 |
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Cell and Tissue Culture
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34 |
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Safety Equipment (Primary Containment)
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35 |
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Biosafety Cabinets (BSC)
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35 |
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What is a Biosafety Cabinet?
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35 |
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When Must I Use a BSC?
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36 |
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Open Flames in a BSC
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36 |
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Decontamination and Ultraviolet Lights in a BSC
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36 |
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Annual Certification Testing
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37 |
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Moving or Repairs
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37 |
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Purchasing and Installing a New BSC
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37 |
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Facility Design (Secondary Containment)
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38 |
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E. Disposal and Disinfection of Biohazardous Materials
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39 |
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University Policies
|
39 |
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Supplies
|
39 |
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What If I Do Not Have Waste Handling Facilities?
|
39 |
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Autoclaves
|
39 |
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Elements Required for Effective Autoclave Use
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39 |
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Autoclave Safety
|
41 |
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Pressure Vessel Monitoring
|
41 |
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Chemical Disinfectants
|
42 |
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Choosing a Chemical Disinfectant
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42 |
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Types of Chemical Disinfectants
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43 |
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Inactivation, Verification, and Biocontainment of Infectious Agents and Toxins
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46 |
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Prion Inactivation and Biocontainment Procedures
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46 |
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USDA Recommendations for Inactivation of Prions Affecting Livestock
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46 |
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BMBL Recommendations for Inactivation of Prions Affecting Humans
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46 |
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Precautions in Using NaOH or Sodium Hypochlorite Solutions in Autoclaves
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47 |
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Biocontainment and Working Procedures
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47 |
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F. Biohazard Spill Clean-up
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48 |
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Biohazard Spill Kit
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48 |
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Biohazard Spill Response Procedure
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49 |
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G. Transporting and Shipping Biohazardous Materials
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51 |
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On-Campus Transport of Biohazardous Materials
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51 |
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Off-Campus Transport of Biohazardous Materials by Commercial Carriers
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51 |
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Permit Requirements
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52 |
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Animals, Plants, Introduction of Genetically Modified Organisms
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52 |
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Human Pathogens or Biological Toxins
|
52 |
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CDC Importation Permits for Etiologic Agents
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52 |
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Select Agents and Toxins
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53 |
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Packaging and Paperwork Requirements
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54 |
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Off-Campus Transport of Biohazardous Materials by Non-Commercial Routes
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55 |
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H. Biological Material Inventory and Biohazardous Materials Security
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57 |
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Bioinventory
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57 |
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Biosecurity
|
57 |
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Non-discrimination Statement
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58 |