Biosafety Manual

Directory of Service and Emergency Providers 3
A. Introduction 7
Definition of Biohazardous Materials 7
Purpose 7
Responsibilities 8
Iowa State University (ISU) 8
Environmental Health and Safety (EH&S) 8
Supervisors 8
Personnel 8
Institutional Biosafety Committee (IBC) 8
B. Recombinant or Synthetic Nucleic Acid Molecules, Human, Animal, and Plant Pathogens, Biological Toxins: Institutional Biosafety Committee (IBC) 10
C. Medical Survelliance 12
Workplace Exposure Assessment 12
Exposure Monitoring 12
Vaccinations and Testing 13
Exposure to Biohazardous Materials 13
Work-Related Injuries, Illnesses and Exposures 14
Reporting 14
D. Biosafety Practices and Procedures 16
Work Practices (First Line Of Defense) 16
Laboratory Biosafety Level Criteria 16
Biosafety Level 1 (BSL–1) Minimum Criteria 18
Biosafety Level 2 (BSL–2) Minimum Criteria 19
Biosafety Level 3 (BSL–3) Minimum Criteria 20
Biosafety Level 4 (BSL–4) 21
Laboratory Decommissioning 22
Training and Education 22
Signs and Labeling 23
Security 23
Personal Protective Equipment (PPE) 24
Laboratory Practice and Technique 26
Pipetting 27
Centrifugation 28
Using Needles, Syringes, and Other Sharps 28
Blending, Grinding, Sonicating, Lyophilizing, and Freezing 29
Open Flames 29
Flow Cytometry 30
Evaluating Laboratory Safety 30
Animal Handling 31
Animals on Campus 31
Animals in the Field 32
Arthropod Research 32
Arthropod Containment Levels 33
Cell and Tissue Culture 34
Safety Equipment (Primary Containment) 35
Biosafety Cabinets (BSC) 35
What is a Biosafety Cabinet? 35
When Must I Use a BSC? 36
Open Flames in a BSC 36
Decontamination and Ultraviolet Lights in a BSC 36
Annual Certification Testing 37
Moving or Repairs 37
Purchasing and Installing a New BSC 37
Facility Design (Secondary Containment) 38
E. Disposal and Disinfection of Biohazardous Materials 39
University Policies 39
Supplies 39
What If I Do Not Have Waste Handling Facilities? 39
Autoclaves 39
Elements Required for Effective Autoclave Use 39
Autoclave Safety 41
Pressure Vessel Monitoring 41
Chemical Disinfectants 42
Choosing a Chemical Disinfectant 42
Types of Chemical Disinfectants 43
Inactivation, Verification, and Biocontainment of Infectious Agents and Toxins 46
Prion Inactivation and Biocontainment Procedures 46
USDA Recommendations for Inactivation of Prions Affecting Livestock 46
BMBL Recommendations for Inactivation of Prions Affecting Humans 46
Precautions in Using NaOH or Sodium Hypochlorite Solutions in Autoclaves 47
Biocontainment and Working Procedures 47
F. Biohazard Spill Clean-up 48
Biohazard Spill Kit 48
Biohazard Spill Response Procedure 49
G. Transporting and Shipping Biohazardous Materials 51
On-Campus Transport of Biohazardous Materials 51
Off-Campus Transport of Biohazardous Materials by Commercial Carriers 51
Permit Requirements 52
Animals, Plants, Introduction of Genetically Modified Organisms 52
Human Pathogens or Biological Toxins 52
CDC Importation Permits for Etiologic Agents 52
Select Agents and Toxins 53
Packaging and Paperwork Requirements 54
Off-Campus Transport of Biohazardous Materials by Non-Commercial Routes 55
H. Biological Material Inventory and Biohazardous Materials Security 57
Bioinventory 57
Biosecurity 57
Non-discrimination Statement 58

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