20 Biosafety Manual annually. • Certified biosafety cabinets, located away from high-traffic areas and drafts, are used for procedures with a potential for creating infectious aerosols or splashes and for handling large volumes of pathogenic materials. • Centrifuges having sealed rotor heads or centrifuge safety cups are used. • Personnel wear safety glasses, goggles, or a face shield during operations posing potential for splashes or aerosols. • Protective clothing (such as lab coats) is kept inside the laboratory. • Appropriate gloves are worn when handling hazardous materials. • Laboratory personnel are enrolled in the respiratory protection program when warranted by risk assessment. • Doors are self-closing and lockable. • An eyewash station is located within the laboratory. • Illumination is adequate for all activities, avoiding reflections and glare that could affect vision. • Vacuum lines are protected with liquid disinfectant traps. • Potentially infectious materials must be placed in a durable, leak- proof container during collection, handling, storage, and transport . Biosafety Level 3 (BSL–3) Minimum Criteria • BSL-2 minimum criteria are followed. • Laboratory is accessed by a double door access zone with self- closing doors and sealed penetrations. • Laboratory doors remain closed at all times. • Every consideration has been given to the physical construction of the BSL-3 laboratory (for example sealed penetrations, smooth walls, sealed joints, and cove bases). • Depending on the biohazardousmaterials used or special handling conditions, HEPA (High Efficiency Particulate Air) filtration may be required for air exiting the room to the outdoors. • Directional airflow, flowing from clean areas to contaminated areas, is provided. • All experimental manipulations are done inside a certified biosafety cabinet (primary containment). • Every consideration is given to alternative forms of needles or glassware to prevent sharps injuries.