Biosafety Manual

20 Biosafety Manual immunizations if available. • A biosafety manual and SOPs are written to incorporate specific biosafety precautions appropriate to the laboratory. • Procedures for handling a spill or accident, including required followup and documentation, are in place and available to lab personnel. • Exhaust HEPAfilters or equipment containing HEPAfilters, including biosafety cabinets and cage racks, must be certified annually. • Certified biosafety cabinets, located away from high-traffic areas and drafts, are used for procedures with a potential for creating infectious aerosols or splashes and for handling large volumes of pathogenic materials. • Centrifuges having sealed rotor heads or centrifuge safety cups are used. • Personnel wear safety glasses, goggles, or a face shield during operations posing potential for splashes or aerosols. • Protective clothing (such as lab coats) is kept inside the laboratory. • Appropriate gloves are worn when handling hazardous materials. • Laboratory personnel are enrolled in the respiratory protection programwhenwarrantedby riskassessment.The riskassessment considers whether respiratory protection is needed for the work with hazardous materials. If needed, relevant staff are enrolled in a properly constituted respiratory protection program. • Doors are self-closing and lockable. • Illumination is adequate for all activities and avoids reflections and glare that could impede vision. • Vacuum lines are protected with liquid disinfectant traps. • Potentially infectious materials must be placed in a durable, leakproof container during collection, handling, storage, and transport. Biosafety Level 3 (BSL–3) Minimum Criteria • BSL-2 minimum criteria are followed. • Access to the laboratory is through two consecutive self-closing doors. • Laboratory doors are lockable in accordance with institutional policies. • Every consideration has been given to the physical construction of the BSL-3 laboratory (for example sealed penetrations, smooth walls, sealed joints, and cove bases). • Depending on the biohazardousmaterials used or special handling conditions, HEPA (High Efficiency Particulate Air) filtration may be